Improved Analysis of DNA Short Tandem Repeats with Time-of-Flight Mass Spectrometry Final Report

The report includes: (1) Executive Summary (descriptions of Gene Trace Systems and Mass Spectrometry, Short Tandem Repeats and Single Nucleotide Polymorphisms and Papers Published and Presentations Made); (2) Project Description and Grants; (3) Scope and Methodology (Assay Development and Primer Testing, Sample Cleanup and Mass Spectrometry, Sample Genotyping, Comparison Tests With ABI 310); (4) Results and Discussion for STR Analysis by Mass Spectrometry (Marker Selection and Feasibility Studies, Multiplex STR Work, Comparison Tests Between ABI 310 and Mass Spec Results, PCR Issues, Analytical Capabilities of Mass Spec); (5) Results and Discussion for Multiplex SNPs (Mitochondrial DNA Work, Y Chromosome Work); and (6) Conclusions and Implications of Findings. Notes, references, figures, tables