Forensic DNA: Non-Human DNA

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Description

Forensic investigations can be aided by association of biological material from animals, plants, or microbes to a victim or suspect. For example, domestic animals such as cats and dogs live in human habitats and may deposit hair that can be used to associate a suspect with a particular crime scene or victim. Thus, efforts are underway to improve genetic marker systems and assays for cat and dog DNA. Sources of marijuana may also be linked through the power of DNA testing.

Entomological evidence also can be used to investigate crimes. For example, insects found on a homicide victim can help determine time of death.

Research Projects

The table below presents awards funded both prior to and under the DNA Initiative, which began in 2004.

Title Awardee Amount Award Number
Extending the Microbial Forensic Toolkit Through Whole Genome Sequencing and Statistical Phylogenomics Baylor College of Medicine 2011-DN-BX-K534 $581,213
Development of a DNA-Based Multi-Species Identification and Quantification Assay University of California, Davis 2008-DN-BX-K288 $538,653
A Rapid, Efficient and Effective Assay to Determine Species Origin in Biological Materials Bode Technology Group, Inc. 2007-DN-BX-K144 $170,212
DNA-Based Identification of Forensically Important Diptera University of Cincinnati 2005-DA-BX-K102 $574,557
Development and Validation of a Standardized Canine STR Panel for Use in Forensic Casework University of California 2004-DN-BX-K007 $310,798
Generating More Precise Postmortem Interval Estimates With Entomological Evidence Michigan State University 2004-DN-BX-K005 $185,001
mtDNA Reference Database for Domestic Dogs George Washington University 2004-DN-BX-K004 $243,144
Plant DNA Typing by Amplified Fragment Length Polymorphism University of New Haven 2001-IJ-CX-K011 $259,868
Development of a PCR-Based Forensic Typing System for Genetic Individualization of Domestic Cats Laboratory for Genomic Research, National Cancer Institute 1999-IJ-CX-A079 $265,570
Development of DNA-Based Identification Techniques for Forensic Entomology, Phase 2 University of Alabama at Birmingham 1999-IJ-CX-0034 $115,366
Development of DNA Identification Techniques for Forensically Important Insects University of California—Berkeley 1997-IJ-CX-0035 $60,000

Research Articles and Reports

Select a title to view an abstract from PubMed database (National Library of Medicine's) unless a different file type is indicated.

Title Authors Publication Year Awardee Award Number
Building a Genetic Reference Database for Dog mtDNA Sequences and SNPs (pdf, 69 pages) Marc W. Allard Final Grant Report 2009 2004-DN-BX-K004 George Washington University
Identification of forensically informative SNPs in the domestic dog mitochondrial control region. Webb KM, Allard MW. J Forensic Sci. 2009 Mar;54(2):289-304. 2009 George Washington University 2004-DN-BX-K004
Mitochondrial genome DNA analysis of the domestic dog: identifying informative SNPs outside of the control region. Webb KM, Allard MW. J Forensic Sci. 2009 Mar;54(2):275-88. 2009 George Washington University 2004-DN-BX-K004
Development and Validation of a Standardized Canine STR Panel for Use in Forensic Casework (pdf, 63 pages) Sree Kanthaswamy Final Grant Report 2009 University of California, Davis 2004-DN-BX-K007
Forensic utility of the mitochondrial hypervariable region 1 of domestic dogs, in conjunction with breed and geographic information Himmelberger AL, Spear TF, Satkoski JA, George DA, Garnica WT, Malladi VS, Smith DG, Webb KM, Allard MW, Kanthaswamy S J Forensic Sci. 2008 Jan;53(1):81-9 2008 University of California, Davis 2004-DN-BX-K007
Generalized Additive Models and Lucilia sericata Growth: Assessing Confidence Intervals and Error Rates in Forensic Entomology Tarone AM, Foran DR J Forensic Sci. 2008 Jul; 53(4):942-8 2008 Michigan State University 2004-DN-BX-K005
Real-time polymerase chain reaction quantification of canine DNA. Evans JJ, Wictum EJ, Penedo MC, Kanthaswamy S. J Forensic Sci. 2007 Jan; 52(1):93-6. 2007 University of California 2004-DN-BX-K007
Validation of a short tandem repeat multiplex typing system for genetic individualization of domestic cat samples. Coomber N, David VA, O'Brien SJ, Menotti-Raymond M. Croat Med J. 2007 Aug; 48(4):547-55. 2007 National Cancer Institute 1999-IJ-CX-A079
Components of developmental plasticity in a Michigan population of Lucilia sericata (Diptera: Calliphoridae). Tarone AM, Foran DR. J Med Entomol. 2006 Sep; 43(5):1023-33. 2006 Michigan State University 2004-DN-BX-K005
Validation of a DNA Method for the Individualization of Plant Evidence (pdf, 19 pages) Heather Miller Coyle, Gary Shutler, Lois Tully, Elaine Pagliaro, Albert Harper Final Grant Report 2005 University of New Haven 2001-IJ-CX-K011
An STR forensic typing system for genetic individualization of domestic cat (Felis catus) samples Menotti-Raymond MA, David VA, Wachter LL, Butler JM, O'Brien SJ J Forensic Sci. 2005 Sep; 50(5):1061-1070. 2005 Laboratory for Genomic Research, National Cancer Institute 1999-IJ-CX-A079
Mitochondrial DNA and STR analyses of maggot crop contents: effect of specimen preservation technique. Linville JG, Hayes J, Wells JD. J Forensic Sci. 2004 Mar; 49(2):341-4. 2004 University of Alabama at Birmingham 1999-IJ-CX-0034
An Overview of DNA Methods for the Identification and Individualization of Marijuana Coyle HM, Palmbach T, Juliano N, Ladd C, Lee HC Croatian Medical Journal 2003 44(3):315-321 2003 University of New Haven 2001-IJ-CX-K011
Quantitative polymerase chain reaction-based assay for estimating DNA yield extracted from domestic cat specimens. Menotti-Raymond M, David V, Wachter L, Yuhki N, O'Brien SJ. Croat Med J. 2003 Jun; 44(3):327-31. 2003 Laboratory for Genomic Research, National Cancer Institute 1999-IJ-CX-A079
DNA-based identification of forensically important Chrysomyinae (Diptera: Calliphoridae). Wells JD, Sperling FA. Forensic Sci Int. 2001 Aug 15; 120(1-2):110-5. 2001 University of Alabama at Birmingham 1999-IJ-CX-0034
Human and insect mitochondrial DNA analysis from maggots. Wells JD, Pape T, Sperling FA. J Forensic Sci. 2001 May; 46(3):685-7. 2001 University of Alabama at Birmingham 1999-IJ-CX-0034
DNA-based identification and molecular systematics of forensically important Sarcophagidae (Diptera). Wells JD, Pape T, Sperling FA. J Forensic Sci. 2001 Sep; 46(5):1098-102. 2001 University of Alabama at Birmingham 1999-IJ-CX-0034
Date Modified: October 11, 2012