Forensic DNA: General Tools and Information

Description

A number of general tools are being developed to aid State and local forensic DNA laboratories. Studies have been performed or are ongoing to improve the efficiency of casework, to examine population variation with forensic DNA markers, to evaluate different DNA storage media, to assess capabilities of DNA in ascertaining human pigmentation or time since deposition from crime scene samples, and to examine the costs of blind proficiency testing. In addition, valuable information is being made available to aid forensic practitioners, researchers, and officers of the court through Internet resources such as STRBase (http://www.cstl.nist.gov/biotech/strbase/).

Research Projects

The table below presents awards funded both prior to and under the DNA Initiative, which began in 2004.

Title Awardee Amount Award Number
Automated Multianalyte Screening Tool for Classification of Forensic Samples Advanced Liquid Logic, Inc. 2011-DN-BX-K556 $996,237
Developing an Empirically-Based Ranking Order for Bone Sampling: Examining the Differential DNA Yield Rates between Human Skeletal Elements over Increasing Post Mortem Intervals University of Tennessee Knoxville 2010-DN-BX-K229 $200,316
Evaluating the Use of DNA and RNA Degradation for Estimating the Post-Mortem Interval University of Tennessee 2010-DN-BX-K228 $167,414
Developing a Forensic Resource/Reference On Genetics Knowledge base Yale University 2010-DN-BX-K226 $643,771
Exploring Soil Bacterial Communities for Forensic Applications: A Genomics Approach University of Connecticut 2009-IJ-CX-0021 $20,000
Identification and Separation of Evidence Mixtures Using SNP-Based FISH Techniques and Laser Microdissection The Bode Technology Group, Inc 2009-DN-BX-K250 $320,021
Genome-Wide Forensic DNA Analysis University of Utah 2012-DN-BX-K037 $541,266
Familial DNA Database Search System-Hardware/Software Integration Project City and County of Denver 2012-DN-BX-K036 $447,979
Developmental Validation of a High-Specificity Multiplex Assay for Human Body Fluid Identification Colorado Seminary, which owns the University of Denver 2012-DN-BX-K035 $576,429
Development of Reference Sample DNA Profiling for Databases Using Next Generation Sequencing Technologies University of North Texas Health Science Center 2012-DN-BX-K033 $747,797
Trace DNA from Fingernails: Increasing the Success Rate of Widely Collected Forensic Evidence Michigan State University 2010-DN-BX-K224 $132,046
Effective Long-Term Preservation of Biological Evidence Bode Technology Group, Inc 2010-DN-BX-K193 $353,186
Targeted Non-Destructive Evidence Detection and Collection Bode Technology Group, Inc 2010-DN-BX-K191 $348,175
Molecular Characterization of Trace Biological Evidence for the Optimized Recovery and Analysis of 'Touch DNA' University of Central Florida 2010-DN-BX-K139 $304,657
Identification of Forensically Relevant Fluids and Tissues by Small RNA Profiling University of Central Florida 2009-DN-BX-K255 $328,962
Automated Processing of FTA Samples General Electric Company 2009-DN-BX-K187 $499,967
Application of Raman Spectroscopy for an Easy-to-Use, on-Field, Rapid, Nondestructive, Confirmatory Identification of Body Fluids The Research Foundation of SUNY 2009-DN-BX-K186 $374,395
Predicting the Biological Age of a Bloodstain Donor The University of Central Florida 2009-DN-BX-K179 $300,450
Validation of Highly-Specific Protein Markers for the Identification of Biological Stains University of Denver– Colorado Seminary 2009-DN-BX-K165 $243,427
Automated Processing of Sexual Assault Cases Using Selective Degradation Paternity Testing Corporation 2009-DN-BX-K039 $294,360
Identification and Separation of Same Gender Mixtures of Various Cell Types Using Interphase FISH Techniques and Laser Microdissection Bode Technology Group Inc 2008-IJ-CX-K016 $125,773
Taq Mutants Engineered for Forensics DNA Ploymerase Technology, Inc 2008-DN-BX-K299 $200,621
Development of a DNA-Based Real-Time PCR assay for Identification of Semen, Blood and Saliva Towson University 2008-DN-BX-K137 $170,643
Rapid STR Prescreening of Forensic Samples at the Crime Scene Midwest Research Institute 2008-DN-BX-K012 $498,064
Establishment of a Fast and Accurate Proteomic Method for Body Fluid/Cell Type New York City, Office of the Chief Medical Examiner 2008-DN-BX-K011 $426,080
De-convolution of Body Fluid Mixtures: Cell Type Identification and Single Source Genetic Profiling of Micro-Dissected Cells University of Central Florida 2008-DN-BX-K007 $415,776
Forensic Stain Identification by Real-Time PCR Analysis Vermont Department of Public Safety 2007-DN-BX-K149 $112,481
DNA Profiling of the Semen Donor in Extended Interval Post-Coital Samples University of Central Florida 2007-DN-BX-K148 $271,504
Application of Proteinases for DNA Isolation of Bone Specimens Indiana University 2006-DN-BX-K010 $15,696
Determination of the Age (Time Since Deposition) of a Biological Stain University of Central Florida 2005-MU-BX-K071 $124,753
Improving the Efficiency of DNA Casework Analysis Through Simple, Effective, PCR-Based Screening Methods Vermont Department of Public Safety 2005-DA-BX-K003 $256,000
Graduate Research Fellowship: Bayesian Networks in Forensic Science North Carolina State University 2004-DN-BX-K006 $20,000
Forensic Stain Identification by RT-PCR Analysis Vermont Department of Public Safety 2004-DN-BX-K002 $240,404
DNA Research in Support of Crime Laboratory Improvement University of Connecticut 2003-LP-CX-K026 $2,000,000
Automated STR Mixture Analysis Cybergenetics Co. 2001-IJ-CX-K003 $396,525
SpermPaint Optimization and Validation University of Virginia 2000-IJ-CX-K013 $339,059
SpermPaint Optimization and Validation University of Virginia 2000-IJ-CX-K013 $305,532
Development of New Analytical Buffer Systems for the Separation and Analysis of PCR-Amplified DNA Ohio University 1999-IJ-CX-K014 $113,923
Development of New Analytical Buffer Systems for the Separation and Analysis of PCR-Amplified DNA Ohio University 1999-IJ-CX-K014 $165,278
Forensic DNA Typing Using High-Performance Liquid Chromatography American University 1999-IJ-CX-0033 $54,996
Evaluation of New STR Markers for Forensic Analysis University of Pittsburgh 1997-LB-VX-0009 $220,359
Validation of PCR-Based DNA Typing Databases for Forensic Use University of Texas-Houston 1996-IJ-CX-0023 $96,704
Validation of PCR-Based DNA Typing Data Bases for Forensic Use University of Texas-Houston 1996-IJ-CX-0023 $146,704
Development of Criteria for Model External DNA Proficiency University of Illinois-Chicago 1996-DN-VX-0001 $249,621
Development of Criteria for Model External DNA Proficiency University of Illinois-Chicago 1996-DN-VX-0001 $249,926
Isolation and Characterization of Population-Specific Alleles University of Pittsburgh 1995-IJ-CX-0008 $99,677
Isolation and Characterization of Population-Specific Alleles University of Pittsburgh 1995-IJ-CX-0008 $99,965
Estimation of Population Structure Parameters North Carolina State University 1995-IJ-CX-0007 $25,000
Estimation of Population Structure Parameters North Carolina State University 1995-IJ-CX-0007 $26,249

Research Articles and Reports

Select a title to view an abstract from PubMed database (National Library of Medicine's) unless a different file type is indicated.

Title Authors Publication Year Awardee Award Number
Laser Microdissection as a Technique to Resolve Mixtures and Improve the Analysis of Difficult Evidence Samples (pdf, 96 pages) Robert Driscoll, MFS ; Dane Plaza, BS ; Robert Bever, Ph.D. Final Grant Report 2009 The Bode Technology Group, Inc. 2006-DN-BX-K032
Application of Proteinases for DNA Isolation of Bone Specimens (pdf, 35 pages) Richard C. Li, Ph.D. Final Grant Report 2009 Indiana University 2006-DN-BX-K010
A real-time multiplex SNP melting assay to discriminate individuals. Nicklas JA, Buel E. J Forensic Sci. 2008 Nov;53(6):1316-24. 2009 Vermont Department of Public Safety 2005-DA-BX-K003
Forensic Stain Identification By RT-PCR Analysis (pdf, 130 pages) Tricia Conti and Eric Buel Final Grant Report 2009 Vermont Department of Public Safety 2004-DN-BX-K002
Determination of the Age (Time since Deposition) of a Biological Stain (pdf, 85 pages) Jack Ballantyne Final Grant Report 2009 University of Central Florida 2005-MU-BX-K071
Demonstration of rapid multiplex PCR amplification involving 16 genetic loci. Vallone PM, Hill CR, Butler JM. Forensic Sci Int Genet. 2008 Dec;3(1):42-5. 2008 NIST 2003-IJ-R-029
Demonstration of rapid multiplex PCR amplification involving 16 genetic loci Peter M. Vallone, Carolyn R. Hilla and John M. Butler Forensic Science International: Genetics
Volume 3, Issue 1, December 2008, Pages 42-45
2008 NIST 2003-IJ-R-029
Validation of reduced-scale reactions for the Quantifiler Human DNA kit Westring CG, Kristinsson R, Gilbert DM, Danielson PB. J Forensic Sci. 2007 Sep; 52(5):1035-43. Epub 2007 Aug 6. 2007 University of Denver 2003-IJ-CX-K104
Forensic science. Brettell TA, Butler JM, Almirall JR. Anal Chem. 2007 Jun 15; 79(12):4365-84. Epub 2007 May 17. 2007 NIST 2003-IJ-R-029
Short tandem repeat typing technologies used in human identity testing. Butler, J.M. Biotechniques. 2007 Oct; 43(4):ii-v 2007 NIST 2003-IJ-R-029
Multiplex_QA: an exploratory quality assessment tool for multiplexed electrophoretic assays. Dueler DL, Butler JM. Electrophoresis. 2006 Oct; 27(19):3735-46. 2006 National Institute of Standards and Technology 2003-IJ-R-029
Genetics and genomics of core short tandem repeat loci used in human identity testing. Butler JM. J Forensic Sci 2006 Mar; 51(2):253-65 2006 National Institute of Standards and Technology 2003-IJ-R-025
AutoDimer: a screening tool for primer-dimer and hairpin structures. Vallone PM and Butler JM Biotechniques. 2004 Aug; 37(2):226-31. 2004 National Institute of Standards and Technology 1999-IJ-R-A094
NIST mixed stain study 3: signal intensity balance in commercial short tandem repeat multiplexes. Duewer DL, Kline MC, Redman JW, Butler JM Anal Chem. 2004 Dec 1; 76(23):6928-34 2004 National Institute of Standards and Technology 1999-IJ-R-A094
Constructing STR Multiplex Assays. Butler JM Methods Mol Biol. 2004; 297:53-66. 2004 National Institute of Standards and Technology 1999-IJ-R-A094
The feasibility of external blind DNA proficiency testing. II. Experience with actual blind tests Peterson JL, Crim D, Lin G, Ho M, Chen Y, Gaensslen RE J Forensic Sci Jan 2003, vol. 48, No. 1, 32-40 2003 University of Illinois at Chicago 1996-DN-VX-0001
NIST Mixed Stain Study 3: DNA quantitation accuracy and its influence on short tandem repeat multiplex signal intensity. Kline MC, Duewer DL, Redman JW, Butler JM. Anal Chem. 2003 May 15; 75(10):2463-9. 2003 National Institute of Standards and Technology 1999-IJ-R-A094
The feasibility of external blind DNA proficiency testing. I. Background and findings Peterson JL, Crim D, Lin G, Ho M, Chen Y, Gaensslen RE J Forensic Sci Jan 2003, vol. 48, No. 1, 21-31 2003 University of Illinois at Chicago 1996-DN-VX-0001
SAMP14, a novel, acrosomal membrane-associated, glycosyl phosphatidylinositol - anchored member of the Ly-6/urokinase-type plasminogen activator receptor superfamily with a role in sperm-egg interaction. Shetty J, Wolkowicz MJ, Digilio LC, Klotz KL, Jayes FL, Diekman AB, Westbrook VA, Farris EM, Hao Z, Coonrod SA, Flickinger CJ, Herr JC. J Biol Chem. 2003 Aug 15; 278(33):30506-15. Epub 2003 Jun 04. 2003 University of Virginia 2000-IJ-CX-K013
Extraction and analysis of human nuclear and mitochondrial DNA from electron beam irradiated envelopes. Withrow AG, Sikorsky J, Downs JC, Fenger T. J Forensic Sci. 2003 Nov; 48(6):1302-8. 2003 Marshall University 2001-RC-CX-K002
SLLP1, a unique, intra-acrosomal, non-bacteriolytic, c lysozyme-like protein of human spermatozoa. Mandal A, Klotz KL, Shetty J, Jayes FL, Wolkowicz MJ, Bolling LC, Coonrod SA, Black MB, Diekman AB, Haystead TA, Flickinger CJ, Herr JC. Biol Reprod. 2003 May; 68(5):1525-37. Epub 2002 Nov 27. 2003 University of Virginia 2000-IJ-CX-K013
Allele frequencies for 15 autosomal STR loci on U.S. Caucasian, African American, and Hispanic populations. Butler JM, Schoske R, Vallone PM, Redman JW, Kline MC. J Forensic Sci. 2003 Jul; 48(4):908-11. 2003 National Institute of Standards and Technology 1999-IJ-R-A094
A strategy for examining complex mixtures of deoxyoligonucleotides using ion-pair-reverse-phase high-performance liquid chromatography, matrix-assisted laser desorption ionization time-of-flight mass spectrometry, and informatics. Vallone PM, Devaney JM, Marino MA, Butler JM Anal Biochem. 2002 May 15; 304(2):257-65. 2002 National Institute of Standards and Technology 1999-IJ-R-A094
Effect of sample preparation and pH-mediated sample stacking on the analysis of multiplexed short tandem repeats by capillary electrophoresis. Crivellente F, McCord BR. J Capillary Electrophor. 2002; 7(3-4):73-80. 2002 Ohio University 1999-IJ-CX-K014
Polymerase chain reaction amplification of DNA from aged blood stains: quantitative evaluation of the "suitability for purpose" of four filter papers as archival media. Kline MC, Duewer DL, Redman JW, Butler JM, Boyer DA. Anal Chem. 2002 Apr 15; 74(8):1863-9. 2002 National Institute of Standards and Technology 1999-IJ-R-A094
Capillary electrophoresis as a tool for optimization of multiplex PCR reactions. Butler JM, Ruitberg CM, Vallone PM. Fresenius J Anal Chem. 2001 Feb; 369(3-4):200-5. 2001 National Institute of Standards and Technology 1999-IJ-R-A094
STRBase: a short tandem repeat DNA database for the human identity testing community. Ruitberg CM, Reeder DJ, Butler JM. Nucleic Acids Res. 2001 Jan 1; 29(1):320-2. 2001 National Institute of Standards and Technology 1999-IJ-R-A094
Genotyping of two mutations in the HFE gene using single-base extension and high-performance liquid chromatography. Devaney JM, Pettit EL, Kaler SG, Vallone PM, Butler JM, Marino MA. Anal Chem. 2001 Feb 1; 73(3):620-4. 2001 National Institute of Standards and Technology 1999-IJ-R-A094
Linear mixture analysis: a mathematical approach to resolving mixed DNA samples. Perlin MW, Szabady B. J Forensic Sci. 2001 Nov;46(6):1372-8. 2001 Cybergenetics Co. 2001-IJ-CX-K003
Temperature and pH studies of short tandem repeat systems using capillary electrophoresis at elevated pH. Nock T, Dove J, McCord B, Mao D. Electrophoresis. 2001; 22(4):755-62. 2001 Ohio University 1999-IJ-CX-K014
Quality control of PCR primers used in multiplex STR amplification reactions. Butler JM, Devaney JM, Marino MA, Vallone PM. Forensic Sci Int. 2001 Jun 1; 119(1):87-96. 2001 National Institute of Standards and Technology 1999-IJ-R-A094
World population data for the HLA-DQA1, PM and D1S80 loci with least and most common profile frequencies for combinations of loci estimated following NRC II guidelines. Peterson BL, Su B, Chakraborty R, Budowle B, Gaensslen RE. J Forensic Sci. 2000 Jan;45(1):118-46. 2000 University of Illinois at Chicago 1998-VB-R-018
Date Modified: October 11, 2012