Forensic DNA: Y-Chromosome

On this page find:

Description

The Y chromosome DNA testing enables examination of the male-specific portion of biological evidence. This capability can be especially important in situations where a small amount of male DNA may be recovered in the presence of excess female DNA, such as in sexual assault evidence. Y chromosome analysis can also benefit missing persons investigations as it extends the range of potential reference samples. Since fathers pass their Y chromosome onto their sons unchanged (except for an occasional mutation), all males in a paternal lineage will possess a common Y chromosome haplotype.

A core set of Y chromosome short tandem repeat (Y-STR) loci have been selected for use in human identification applications. The core Y-STR loci were recommended by the SWGDAM Y chromosome subcommittee in 2003 and include the following: DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS438, DYS439, and the multi-copy locus DYS385 a/b. Commercial kits now exist that can co-amplify all of the core Y-STR loci in a single multiplex reaction. Several groups are also exploring additional Y-STR loci that have the potential to improve the power of discrimination for Y-STR testing.

Research Projects

The table below presents awards funded both prior to and under the DNA Initiative, which began in 2004.

TitleAwardeeAmountAward Number
Y Chromosome Whole Genome Analysis Strategies: Improved Detection of Male DNAUniversity of Central Florida2007-DN-BX-K147$324,705
Forensic Applications of Y-Chromosome STR's and SNP'sArizona Board of Regents, University of Arizona2000-IJ-CX-K006$430,140
Forensic Applications of Y-Chromosome STR's and SNP'sArizona Board of Regents, University of Arizona2000-IJ-CX-K006$551,418
Validation of Y Chromosome STR Multiplexes for Operational UseUniversity of Central Florida1998-IJ-CX-K003$519,964
Development of the Human Y Chromosome as a Forensic ToolUniversity of Arizona1997-LB-VX-0010$147,529

Research Articles and Reports

Select a title to view an abstract from PubMed database (National Library of Medicine's) unless a different file type is indicated.

TitleAuthorsPublicationYearAwardeeAward Number
The golden gene (SLC24A5) differentiates US sub-populations within the ethnically admixed Y-SNP haplogroupsSims LM, Ballantyne JLeg Med (Tokyo). 2008 Mar; 10(2):72-7. Epub 2007 Aug 272008University of Central Florida1998-IJCX-K003
Y-STR profiling in extended interval (> or = 3 days) postcoital cervicovaginal samplesMayntz-Press KA, Sims LM, Hall A, Ballantyne JJ Forensic Sci. 2008 Mar;53(2):342-82008University of Central Florida1998-IJCX-K003
A rare Y chromosome missense mutation in exon 25 of human USP9Y revealed by pyrosequencingSims LM, Ballantyne JBiochem Genet. 2008 Apr; 46(3-4):154-61. Epub 2008 Jan 202008University of Central Florida1998-IJCX-K003
Analysis of mutations in father-son pairs with 17 Y-STR lociDecker, A.E., Kline, M.C., Redman, J.W., Reid, T.M., Butler, J.M.FSI Genetics (2008) 2(3):e31-e35.2008NIST2003-IJ-R-029
Sub-populations within the major European and African derived haplogroups R1b3 and E3a are differentiated by previously phylogenetically undefined Y-SNPs.Sims LM, Garvey D, Ballantyne J.Hum Mutat. 2007 Jan; 28(1):97.2007University of Central Florida1998-IJCX-K003
The impact of additional Y-STR loci on resolving common haplotypes and closely related individualsA.E. Decker, M.C. Kline, P.M. Vallone, J.M. ButlerForensic Science International: Genetics June 2007 (Vol. 1, Issue 2, Pages 215-217)2007NIST2003-IJ-R-029
Population data for a novel, highly discriminating tetra-local Y-chromosome short tandem repeat: DYS503.Hanson EK, Ballantyne J.J Forensic Sci. 2007 Mar; 52(2):498-9.2007University of Central Florida1998-IJCX-K003
Performance characteristics of commercial Y-STR multiplex systems.Mayntz-Press KA, Ballantyne J.J Forensic Sci. 2007 Sep; 52(5):1025-34. Epub 2007 Aug 6.2007University of Central Florida1998-IJCX-K003
An ultra-high discrimination y chromosome short tandem repeat multiplex DNA typing system.Hanson EK, Ballantyne J.PLoS ONE. 2007 Aug 1; 2(1):e688.2007University of Central Florida1998-IJCX-K003
The impact of additional Y-STR loci on resolving common haplotypes and closely related individualsA.E. Decker, M.C. Kline, P.M. Vallone, J.M. ButlerForensic Science International: Genetics June 2007 (Vol. 1, Issue 2, Pages 215-217)2007NIST2003-IJ-R-029
Population data for 48 'Non-Core' Y chromosome STR loci.Hanson EK, Ballantyne J.Leg Med (Tokyo). 2007 Jul; 9(4):221-31. Epub 2007 Feb 22.2007University of Central Florida1998-IJCX-K003
Forensic Applications of Y Chromosome STRs and SNPs (pdf, 51 pages)Michael Hammer and Alan J. ReddFinal Grant Report2006Arizona Board of Regents, University of Arizona2000-IJ-CX-K006
Comprehensive annotated STR physical map of the human Y chromosome: Forensic implicationsHanson EK, Ballantyne JLegal Medicine. 2006 Mar; 8(2):110-202006University of Central Florida1998-IJ-CX-K003
Genetic structure among 38 populations from the United States based on 11 U.S. core Y chromosome STRs.Redd AJ, Chamberlain VF, Kearney VF, Stover D, Karafet T, Calderon K, Walsh B, Hammer MF.J Forensic Sci. 2006 May; 51(3):580-5.2006Arizona Board of Regents, University of Arizona2000-IJ-CX-K006
Testing and evaluation of 43 "noncore" Y chromosome markers for forensic casework applications.Hanson EK, Berdos PN, Ballantyne J.J Forensic Sci. 2006 Nov; 51(6):1298-314.2006University of Central Florida1998-IJ-CX-K003
Allele frequencies for 27 Y-STR loci with U.S. Caucasian, African American, and Hispanic samples.Butler JM, Decker AE, Vallone PM, Kline MC.Forensic Sci Int. 2006 Jan 27; 156(2-3):250-60.2006National Institute of Standards and Technology2003-IJ-R-029
Y-STR concordance study between Y-Plex5, Y-Plex6, Y-Plex12, PowerplexY, Y-Filer, MPI, and MPII.Gross AM, Berdos P, Ballantyne JJ Forensic Sci. 2006 Nov; 51(6):1423-8.2006University of Central Florida1998-IJ-CX-K003
U.S. population data for the multi-copy Y-STR locus DYS464.Butler JM, Schoske R.J Forensic Sci. 2005 Jul; 50(4):975-72005National Institute of Standards and Technology2003-IJ-R-029
DNA Commission of the International Society of Forensic Genetics (ISFG): an update of the recommendations on the use of Y-STRs in forensic analysis.Gusmao L, Butler JM, Carracedo A, Gill P, Kayser M, Mayr WR, Morling N, Prinz M, Roewer L, Tyler-Smith C, Schneider PM.Int J Legal Med. 2005 Aug 26;:1-102005National Institute of Standards and Technology2003-IJ-R-029
Chromosomal duplications along the Y-chromosome and their potential impact on Y-STR interpretation.Butler JM, Decker AE, Kline MC, Vallone PMJ Forensic Sci. 2005 Jul; 50(4):853-9.2005National Institute of Standards and Technology2003-IJ-R-029
SWGDAM developmental validation of a 19-locus Y-STR system for forensic casework.Daniels DL, Hall AM, Ballantyne J.J Forensic Sci. 2004 Jul; 49(4):668-83.2004University of Central Florida1998-IJ-CX-K003
High-throughput Y-STR typing of U.S. populations with 27 regions of the Y chromosome using two multiplex PCR assays.Schoske R, Vallone PM, Kline MC, Redman JW, Butler JM.Forensic Sci Int. 2004 Jan 28; 139(2-3):107-21.2004National Institute of Standards and Technology1999-IJ-R-A094
Multiplexed assays for evaluation of Y-SNP markers in U.S. populations. (pdf, 3 pages)Vallone, P.M. and Butler, J.M.Progress in Forensic Genetics 10, Elsevier Science: Amsterdam, The Netherlands, International Congress Series 2004; 1261, 85-87. 2004National Institute of Standards and Technology1999-IJ-R-A094
A highly discriminating 21 locus Y-STR "megaplex" system designed to augment the minimal haplotype loci for forensic casework.Hanson EK, Ballantyne J.J Forensic Sci. 2004 Jan; 49(1):40-51.2004University of Central Florida1998-IJ-CX-K003
Duplication of DYS19 flanking regions in other parts of the Y chromosome.Butler JM and Schoske RInt J Legal Med. 2004 Jun; 118(3):178-832004National Institute of Standards and Technology1999-IJ-R-A094
Y-SNP typing of U.S. African American and Caucasian samples using allele-specific hybridization and primer extension.Vallone PM, Butler JM.J Forensic Sci. 2004 Jul; 49(4):723-32.2004National Institute of Standards and Technology1999-IJ-R-A094
Novel Y-STR typing strategies reveal the genetic profile of the semen donor in extended interval post-coital cervicovaginal samples.Hall A, Ballantyne J.Forensic Sci Int. 2003 Sep 9; 136(1-3):58-722003University of Central Florida1998-IJ-CX-K003
Highly multiplexed assays for measuring polymorphisms on the Y-chromosome (pdf, 5 pages)Butler HM, Schoske R, Vallone PMProgress in Forensic Genetics 9 (Brinkmann, B. and Carracedo, A., eds.), Elsevier Science: Amsterdamn, The Netherlands, International Congress Series 1239, pp. 301-305 2003 2003National Institute of Standards and Technology1999-IJ-R-A094
Recent developments in Y-short tandem repeat and Y-single nucleotide polymorphism analysis (pdf, 21 pages)Butler JMForensic. Sci. Rev. 2003 15:91-1112003National Institute of Standards and Technology1999-IJ-R-A094
The development of an 18-locus Y-STR system for forensic casework.Hall A, Ballantyne J.Anal Bioanal Chem. 2003 Aug; 376(8):1234-46.2003University of Central Florida1998-IJ-CX-K003
Multiplex PCR design strategy used for the simultaneous amplification of 10 Y chromosome short tandem repeat (STR) loci.Schoske R, Vallone PM, Ruitberg CM, Butler JM.Anal Bioanal Chem. 2003 Feb; 375(3):333-43.2003National Institute of Standards and Technology1999-IJ-R-A094
Forensic value of 14 novel STRs on the human Y chromosome.Redd AJ, Agellon AB, Kearney VA, Contreras VA, Karafet T, Park H, de Knijff P, Butler JM, Hammer MF.Forensic Sci Int. 2002 Dec 4; 130(2-3):97-111.2002Arizona Board of Regents, University of Arizona2000-IJ-CX-K006
A novel multiplex for simultaneous amplification of 20 Y chromosome STR markers.Butler JM, Schoske R, Vallone PM, Kline MC, Redd AJ, Hammer MF.Forensic Sci Int. 2002 Sep 10; 129(1):10-24.2002Arizona Board of Regents, University of Arizona2000-IJ-CX-K006
Locus-specific brackets for reliable typing of Y-chromosome short tandem repeat markers.Butler JM, Appleby JE, Duewer DL.J Forensic Sci. 2000 Jan;45(1):118-46.2000National Institute of Standards and Technology2003-IJ-R-029
Date Modified: October 11, 2012